Objective To investigate the clinical effect of "experienced ten acupoints" combined with electro-acupuncture in the treatment of post-stroke qi deficiency and constipation. Methods Sixty patients with post-stroke qi deficiency and constipation were selected as research objects, and were divided into control group and treatment group according to random number table method, with 30 cases in each group. The control group received basic treatment + routine acupuncture therapy for constipation, and the treatment group received basic treatment + "experenced ten acupoints" combined with electro-acupuncture intervention.Compared the complete resolution time of symptoms such as dry hard stools and incompletely defecation between two groups of patients, as well as the stroke syndrome score and the Patient Assessment of Constipation-Quality of Life (PAC-QOL) score before and 1 day after intervention, and the short-term (1 month after intervention) and long-term (6 months after intervention) intervention efficacy. Results After intervention, the resolution time of dry hard stools and incompletely defecation in the treatment group was shorter than that in the control group. The scores of stroke syndrome and PAC-QOL in the two groups were lower than those before intervention, which were lower in the treatment group than in the control group (all P<0.05). The total effective rate of short-term intervention and long-term intervention in the treatment group were higher than those in the control group (33.33% vs. 6.67%, 93.33% vs. 70.00%) (all P<0.05). Conclusion The combination of "experienced ten acupoints" and electro-acupuncture in the treatment of patients with post-stroke qi deficiency constipation can significantly accelerate the resolution of symptoms such as dry hard stools and incompletely defecation, regulate stroke syndrome, improve short-term and long-term efficacy, and improve the patients' quality of life.