Objective To investigate the clinical efficacy and safety of robot-assisted laparoscopic partial nephrectomy for the treatment of duplex kidney in pediatric patients. Methods A retrospective analysis was conducted on the clinical data of 4 children with duplex kidneys who underwent robotic surgery in the First Affiliated Hospital of Guangxi Medical University from February 2022 to August 2023. Diagnosed via urography and renal radionuclide scanning, all patients presented with duplex kidneys and renal function of less than 10%. Among them, there was 1 male and 3 females, whose ages spanned from 12 to 28 months. Moreover, 2 cases were characterized as complete duplex kidneys, while the remaining 2 cases were incomplete duplex kidneys. The operative duration, intraoperative blood loss, and treatment outcomes of the four pediatric patients were collected and analyzed. Results For all four cases, the surgeries were successfully carried out using the robot-assisted laparoscopic surgery system. The operation time was 109 to164 minutes, with an average of 136 minutes. The intraoperative blood loss was 5 to 130 mL, with an average of 43.8 mL. The postoperative hospital stay was 4 to 8 days, with an average of 5.8 days. No postoperative complications such as urinary leakage, infection, or intestinal obstruction were observed. All patients were under follow-up for 12 to 22 months, during which the renal function of the affected side was regularly examined. Half a year after the operation, 1 case showed a decline in renal function, but a reexamination after one year of follow-up revealed an improvement in the renal function of the affected side. As for the other three patients, their renal functions improved remarkably after the operation compared with that before operation. Conclusion Robot-assisted laparoscopic partial nephrectomy is a safe, reliable, and effective approach for treating duplex kidneys in pediatric patients. It excels particularly in detailed anatomical dissection and enabling swift recovery, thus offering a novel alternative for the surgical management of pediatric duplex kidneys.