ObjectiveTo compare the modification effect between collagen hydrogel and matrigel as surface coating of scaffold materials for bone tissue engineering. MethodsThe alkaline phosphatase (ALP) activity, hitological staining and mRNA level of osteogenic specific genes were analyzed in the primary osteoblasts, then the effects of collagen hydrogel and matrigel on osteogenic differentiation were investigated. ResultsIn the collagen group and matrigel group, the cell vitality and proliferative capacity increased, the osteogenic related genes and proteins including ALP, osteocalcin, osteopontin and alpha1 type I collagen were up-regulated. Coating with the collagen hydrogel promotion was more effective in promoting the cell growth and osteogenic differentiation compared to coating with the matrigel. ConclusionCoating with the collagen hydrogel is more beneficial to osteogenic differentiation and is more suitable for improvement of cell scaffold compared to coating with matrigel, and is recommended as as surface coating of scaffold materials for bone tissue engineering.

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